Dr. Stephan Menzel

ZOOM Meeting

January 27th, 2021

1 pm

Chair: Prof. Nina Rohringer



We are looking forward to the talk of Dr Stephan Menzel (Institute of Immunology, UKE) who will present his COVID-19-related research in his talk entitled "Characterization of SARS-CoV-2 specific antibody response by flow cytometry".


We developed a flow cytometry-based assay to detect SARS-CoV-2 specific antibodies in human sera. Current commercial antibody detection tools are based on recombinant proteins such as the spike (S) protein, the isolated receptor binding domain (RBD) or the nucleocapsid (N) protein from SARS-CoV-2. This requires to express, purify and store the recombinant proteins and might lead to problems regarding availability and/or protein quality as well as slow adaptability to new proteins or mutations. We use plasmids encoding for SARS-CoV-2 proteins or protein fragments to transfect HEK cells. Transfected cells that express these proteins are incubated with serum and bound antibodies are detected by anti-human secondary antibodies by flow cytometry. Using plasmids, we are able to easily diversify our approach to different proteins or fragments, strains or mutants without the need to purify proteins. Flow cytometry allows for separate analyses of antibody isotypes and multiplexing samples to analyze antibody response to several proteins at once with very low sample input and overall costs.


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